simvastatin  (LKT Laboratories)

Journal: Scientific Reports

Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro

doi: 10.1038/s41598-024-62615-w

Figure Lengend Snippet: Effect of statins on size and compactness of spheroids. ( a ) ADMSC stem cells, ( b ) pancreatic cancer MiaPaCa-2 cells, concentration of statins—20 µM, Ctr—methanol treated spheroids, P—pravastatin, R—rosuvastatin, L—lovastatin, F—fluvastatin, A—atorvastatin, Pi—pitavastatin, C—cerivastatin, S—simvastatin. Statins were added once, after spheroid formation, 10 weeks ( a ) or 3.5 weeks ( b ) after inoculation. Experiment was carried out in biological dodecaplicates.

Article Snippet: In all experiments, pure forms (≥ 98%) of the following statins were used: atorvastatin (A7658), lovastatin (M1687), simvastatin (S3449), fluvastatin (F4482), cerivastatin (C1668), pravastatin (P6801), rosuvastatin (R5974), and pitavastatin (P3576) (LKT Laboratories, USA).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro

doi: 10.1038/s41598-024-62615-w

Figure Lengend Snippet: Effect of statins on the spheroid formation. ( a ) ADMSC stem cells, ( b ) pancreatic cancer MiaPaCa-2 cells, concentration of statins—20 µM, Ctr methanol treated spheroids, P —pravastatin, R —rosuvastatin, L —lovastatin, F —fluvastatin, A —atorvastatin, Pi —pitavastatin, C —cerivastatin, S —simvastatin. Statins were added once, 24 h after cell inoculation. Experiment was carried out in biological dodecaplicates.

Article Snippet: In all experiments, pure forms (≥ 98%) of the following statins were used: atorvastatin (A7658), lovastatin (M1687), simvastatin (S3449), fluvastatin (F4482), cerivastatin (C1668), pravastatin (P6801), rosuvastatin (R5974), and pitavastatin (P3576) (LKT Laboratories, USA).

Techniques: Concentration Assay

Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: Estimation of Fraction Metabolized by Cytochrome P450 Enzymes Using Long-Term Cocultured Human Hepatocytes.

doi: 10.1124/dmd.121.000765

Figure Lengend Snippet: Fig. 2. Probe CYP substrate or specific metabolite concentrations over time in the absence and presence of the selective CYP inhibitors in human long-term cocultured hepatocytes. The effects of selective CYP inhibitors furafylline (CYP1A2, 20 mM), ticlopidine (20 mM; CYP2B6), montelukast (40 mM; CYP2C8), sulfaphenazole (40 mM; CYP2C9), NBPB (3 mM; CYP2C19), quinidine (5 mM; CYP2D6), and itraconazole (3 mM; CYP3A4) on probe CYP marker activities over 4-days incubation time in human cocultured hepatocytes was illustrated: (A) tacrine (CYP1A2), (B) bupropion (CYP2B6), (C) repaglinide (CYP2C8), (D) diclofenac (CYP2C9), (E) dex- tromethorphan (CYP2D6), and (F) midazolam (CYP3A4) and (G) 4’-hydroxymephenytoin formation at 40 mM S-mephenytoin (CYP2C19), respectively. The probe substrate concentration was 1 mM; otherwise noted as above. The probe CYP substrates and inhibitors were applied at time 0 on day 1, and no further multiple

Article Snippet: Montelukast, simvastatin, and tacrine were purchased from Cayman, LKT Laboratories (St. Paul, MN), respectively.

Techniques: Marker, Incubation, Concentration Assay

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Genes involved in the metabolism of lipids and steroids that were affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin; Ato.—atorvastatin; Lov.—lovastatin. Pravastatin and rosuvastatin did not induce any significant change in MiaPaCa-2 cell gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Epigenetic regulator genes that were affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin. Atorvastatin, lovastatin, pravastatin, and rosuvastatin did not induce any significant change in MiaPaCa-2 cells’ gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Genes involved in posttranslational regulation affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin; Ato.—atorvastatin; Lov.—lovastatin. Pravastatin and rosuvastatin did not induce any significant change in MiaPaCa-2 cell gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing, Binding Assay

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Genes involved in the cell cycle and DNA replication affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin; Ato.—atorvastatin; Lov.—lovastatin. Pravastatin and rosuvastatin did not induce any significant change in MiaPaCa-2 cell gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing, Variant Assay, Membrane

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Genes involved in cell death affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin; Ato.—atorvastatin; Lov.—lovastatin. Pravastatin and rosuvastatin did not induce any significant change in MiaPaCa-2 cell gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing

Journal: Molecules

Article Title: Comparison of Transcriptomic Profiles of MiaPaCa-2 Pancreatic Cancer Cells Treated with Different Statins

doi: 10.3390/molecules26123528

Figure Lengend Snippet: Genes involved in cytoskeleton maintenance affected by statin treatment of MiaPaCa-2 cells. Numeric columns display fold changes of the gene expression between cells treated by individual statins at 12 µM concentration for 24 h and untreated cells. Cer.—cerivastatin; Pit.—pitavastatin; Sim.—simvastatin; Flu.—fluvastatin; Ato.—atorvastatin; Lov.—lovastatin. Pravastatin and rosuvastatin did not induce any significant change in MiaPaCa-2 cell gene expression.

Article Snippet: Briefly, pure forms (≥98 %) of all commercially available statins were used: atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, pravastatin, rosuvastatin, and pitavastatin (LKT Laboratories, USA).

Techniques: Gene Expression, Concentration Assay, Expressing